HSF-2 antibody

Antigen: HSF-2

Clonality: Monoclonal (3E2)

Host: Rat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Guinea Pig, Hamster, Monkey, Rabbit, Dog (Canine), Cow (Bovine), Sheep (Ovine), Pig (Porcine)

Application: Western Blotting (WB)

Catalog no.: ABIN361727

Additional Information

Characteristics: Detects an ~69kDa protein in unstressed cells corresponding to the molecular mass HSF2 on SDS PAGE immunoblots.

Gene ID: 3298

Swiss-Prot: Q03933

Immunogen: Purified recombinant mouse HSF2 protein

Format: Liquid

Isotype: IgG  (Matching secondary antibodies)

Clone: 3E2

Description: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF).
Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization.
There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form. Various experiments have suggested that HFS2 may have roles in differentiation and development.
Synonyms: HSTF2

Specificity: Detects an ~69kDa protein in unstressed cells corresponding to HSF2 on SDS-PAGE immunoblots. Species cross-reactivity: Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit. Dog, Bovine, Sheep, Pig.

Sensitivity: 4 µg/mL of SMC-119 was sufficient for detection of HSF2 in 20µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Application Details

Concentration: 1 mg/mL

Purification: Mouse Immunoglobulin

Buffer: PBS pH7.2, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

HSF2 K562 cells HSF1&2 shRNA constructs

Publications

Publications: Murphy, Gorzowski, Sarge et al.: "Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5309-17, 1994 (PubMed).

Sarge, Park-Sarge, Kirby et al.: "Expression of heat shock factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis." in: Biology of reproduction, Vol. 50, Issue 6, pp. 1334-43, 1994 (PubMed).

Rallu, Loones, Lallemand et al.: "Function and regulation of heat shock factor 2 during mouse embryogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2392-7, 1997 (PubMed).

McMillan, Xiao, Shao et al.: "Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7523-8, 1998 (PubMed).

Morimoto: "Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators." in: Genes & development, Vol. 12, Issue 24, pp. 3788-96, 1999 (PubMed).

Jolly, Usson, Morimoto: "Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6769-74, 1999 (PubMed).

Morano, Thiele: "Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signals." in: Gene expression, Vol. 7, Issue 4-6, pp. 271-82, 1999 (PubMed).

Tanaka, Namba, Arai et al.: "Genetic evidence for a protective role for heat shock factor 1 and heat shock protein 70 against colitis." in: The Journal of biological chemistry, Vol. 282, Issue 32, pp. 23240-52, 2007 (PubMed).

Monechi, Fiumalbi, De Monte et al.: "[Investigation on health status of silica exposed workers in "cotto Fiorentino" companies]" in: Giornale italiano di medicina del lavoro ed ergonomia, Vol. 29, Issue 3 Suppl, pp. 736-7, 2008 (PubMed).