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HSF2 antibody

HSF2 Reactivity: Mouse WB, IF, ICC, AA Host: Rat Monoclonal 3E2 unconjugated
Catalog No. ABIN361728
  • Target See all HSF2 Antibodies
    HSF2 (Heat shock factor protein 2 (HSF2))
    Reactivity
    • 74
    • 42
    • 35
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Mouse
    Host
    • 68
    • 14
    • 5
    • 1
    Rat
    Clonality
    • 69
    • 19
    Monoclonal
    Conjugate
    • 38
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This HSF2 antibody is un-conjugated
    Application
    • 75
    • 28
    • 26
    • 24
    • 23
    • 14
    • 13
    • 10
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)
    Specificity
    Detects ~69 kDa.
    Cross-Reactivity
    Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
    Purification
    Protein G Purified
    Immunogen
    Purified recombinant mouse HSF2 protein
    Clone
    3E2
    Isotype
    IgG1
    Top Product
    Discover our top product HSF2 Primary Antibody
  • Application Notes
    • WB (1:250)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Comment

    4 μg/ml of ABIN361727 was sufficient for detection of HSF2 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    HSF2 (Heat shock factor protein 2 (HSF2))
    Alternative Name
    HSF2 (HSF2 Products)
    Synonyms
    AI661205 antibody, HSF 2 antibody, HSTF 2 antibody, HSF3B antibody, HSTF3B antibody, heat shock factor 2 antibody, heat shock transcription factor 2 antibody, Hsf2 antibody, HSF2 antibody
    Background
    HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
    Gene ID
    15500
    NCBI Accession
    NP_001129036
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