HSF2 antibody
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- Target See all HSF2 Antibodies
- HSF2 (Heat shock factor protein 2 (HSF2))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This HSF2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)
- Specificity
- Detects ~69 kDa.
- Cross-Reactivity
- Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
- Purification
- Protein G Purified
- Immunogen
- Purified recombinant mouse HSF2 protein
- Clone
- 3E2
- Isotype
- IgG1
- Top Product
- Discover our top product HSF2 Primary Antibody
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- Application Notes
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- WB (1:250)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- Comment
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4 μg/ml of ABIN361727 was sufficient for detection of HSF2 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- HSF2 (Heat shock factor protein 2 (HSF2))
- Alternative Name
- HSF2 (HSF2 Products)
- Synonyms
- AI661205 antibody, HSF 2 antibody, HSTF 2 antibody, HSF3B antibody, HSTF3B antibody, heat shock factor 2 antibody, heat shock transcription factor 2 antibody, Hsf2 antibody, HSF2 antibody
- Background
- HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
- Gene ID
- 15500
- NCBI Accession
- NP_001129036
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