anti-HSP90 antibody (Heat Shock Protein 90)

Details for Product anti-HSP90 Antibody No. ABIN361732
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Antigen
Synonyms
git10, swo1, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1, 143198_at, 83, 83K HSP, DMHSP82, E(sev)3A, E(sina)2, HSP82, HSP83, ORF1, Su(Raf)3A, anon-EST:Liang-2.53, anon-WO0068693, anon-WO0 ... show more
git10, swo1, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1, 143198_at, 83, 83K HSP, DMHSP82, E(sev)3A, E(sina)2, HSP82, HSP83, ORF1, Su(Raf)3A, anon-EST:Liang-2.53, anon-WO0068693, anon-WO0140519.209, clone 2.53, en(lz)3C/4C, hsp84, l(3)j5C2, ms(3)08445, stc, DmelCG1242, CG1242, 86kDa, 89kDa, AL024080, AL024147, Hsp86-1, Hsp89, Hsp90, Hspca, hsp4, Hsp86, EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2, hsp86, hsp89, hsp90, hsp90a, hspc1, hspca, hspn, lap2, D6S182, HSP84, HSP90B, HSPC2, HSPCB show less
Reactivity
Human, Mouse (Murine), Rabbit, Rat (Rattus)
(251), (160), (144), (95), (72), (22), (22), (22), (20), (20), (19), (19), (19), (19), (19), (19), (19), (13), (7), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Mouse
(189), (100), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This HSP90 antibody is un-conjugated
(11), (11), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (7), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(260), (173), (172), (155), (127), (120), (24), (9), (2), (2)
Pubmed 7 references available
Quantity 200 μg
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Immunogen Ah receptor (Aryl hydrocarbon receptor)
Clone 8D3
Specificity Detects 90 kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2α (eIF-2α) kinase (HRI).
Sensitivity Goat anti-mouse IgM was used to bind 25 myl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 myl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including hsp90, hsp70 and Hop.
Purification PEG Purified
Alternative Name HSP90 (HSP90 Antibody Abstract)
Background HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7).
Cellular Localization: Cytoplasm | Melanosome
Gene ID 3326
NCBI Accession NP_031381
UniProt P08238
Research Area Heat Shock Proteins
Application Notes Recommended Dilution: ICC/IF (1:100), IP (1:1000)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Heat Shock Protein 90 (HSP90) antibody Hsp90 complex (8D3) isolation, IP (rabbit reticulocyte lysate) SDS PAGE Coomassie.
Background publications Arlander, Eapen, Vroman et al.: "Hsp90 inhibition depletes Chk1 and sensitizes tumor cells to replication stress." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52572-7, 2003 (PubMed).

Pearl, Prodromou: "Structure, function, and mechanism of the Hsp90 molecular chaperone." in: Advances in protein chemistry, Vol. 59, pp. 157-86, 2002 (PubMed).

Pratt: "The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors." in: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), Vol. 217, Issue 4, pp. 420-34, 1998 (PubMed).

Pratt, Toft: "Steroid receptor interactions with heat shock protein and immunophilin chaperones." in: Endocrine reviews, Vol. 18, Issue 3, pp. 306-60, 1997 (PubMed).

Uma, Hartson, Chen et al.: "Hsp90 is obligatory for the heme-regulated eIF-2alpha kinase to acquire and maintain an activable conformation." in: The Journal of biological chemistry, Vol. 272, Issue 17, pp. 11648-56, 1997 (PubMed).

Perdew: "Association of the Ah receptor with the 90-kDa heat shock protein." in: The Journal of biological chemistry, Vol. 263, Issue 27, pp. 13802-5, 1988 (PubMed).

Dalman, Bresnick, Patel et al.: "Direct evidence that the glucocorticoid receptor binds to hsp90 at or near the termination of receptor translation in vitro." in: The Journal of biological chemistry, Vol. 264, Issue 33, pp. 19815-21, 1989 (PubMed).

Validation Images
Catalog No. ABIN361732
497.20 $
Plus shipping costs $45.00
Quantity
Price
200 μg
497.20 $

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