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HSP70 antibody

HSP70 Reactivity: Human WB, IHC, IF, IP, ICC, AA Host: Mouse Monoclonal 2A4 unconjugated
Catalog No. ABIN361734
  • Target See all HSP70 Antibodies
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reactivity
    • 318
    • 197
    • 163
    • 71
    • 60
    • 53
    • 52
    • 52
    • 36
    • 31
    • 29
    • 29
    • 28
    • 23
    • 20
    • 18
    • 15
    • 13
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 206
    • 145
    • 13
    • 11
    • 11
    Mouse
    Clonality
    • 228
    • 157
    Monoclonal
    Conjugate
    • 108
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    This HSP70 antibody is un-conjugated
    Application
    • 370
    • 159
    • 139
    • 121
    • 119
    • 109
    • 97
    • 66
    • 62
    • 61
    • 41
    • 32
    • 22
    • 21
    • 21
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Antibody Array (AA)
    Specificity
    Detects ~70 kDa. May detect HSP70, HSC70, and HSP72.
    Cross-Reactivity
    Amphibian, Chicken, Drosophila melanogaster, Fish, Human, Mouse, Rat, Saccharomyces cerevisiae
    Purification
    PEG Purified
    Immunogen
    Human recombinant HSP70 overexpressed in E.coli
    Clone
    2A4
    Isotype
    IgM
    Top Product
    Discover our top product HSP70 Primary Antibody
  • Application Notes
    • WB (1:5000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.2 μg/ml of ABIN361733 was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgM:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Cases, Joseph, Obry, Santin, Ben-Yacoub, Pâques, Amsellem-Levera, Bribian, Simonutti, Augustin, Debeir, Sahel, Christ, de Castro, Lehéricy, Cosette, Kozyraki: "Foxg1-Cre Mediated Lrp2 Inactivation in the Developing Mouse Neural Retina, Ciliary and Retinal Pigment Epithelia Models Congenital High Myopia." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129518, (2016) (PubMed).

  • Target
    HSP70 (Heat Shock Protein 70 (HSP70))
    Alternative Name
    HSP70 (HSP70 Products)
    Synonyms
    APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
    Background
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
    Gene ID
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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