Heat Shock Protein 9 (HSPA9) antibody

Antigen: Heat Shock Protein 9 (HSPA9)

Synonyms: Hspa9a, HSPA9B, MGC127342

Clonality: Monoclonal (S52A-42)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN361740

Additional Information

Characteristics: Detects ~75kDa.

Alternative name: Grp75

Gene ID: 3313

Swiss-Prot: P38646

Immunogen: Peptide immunogen to SALM2

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S52A-42

Description: Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible. Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle . Related forms may also be found in the cytosol or on the surface of the extracellular membrane. Other Grp75 functions include its ability to inactivate the tumor suppressor p53. Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation. Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts. And finally, like its E.coli homolog DnaK, GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone .
Synonyms: Hsc74, Hsp74, HspA9, Hspa9a, HspA9B, Mortalin 2, MOT2, PBP74

Specificity: ~75kDa. Species cross-reactivity: Human, mouse, rat, other species not yet tested.

Sensitivity: 1µg/ml was sufficient for detection of Grp75 in 10µg of heat shock HeLa lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Application Details

Application Notes: 1:1000 (colorimetric) (WB)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.2, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Signaling, Heat Shock Proteins

Restrictions: For Research Use only

Images

Grp75(S52A-42) human cell lines mix