DNA Damage antibody

Antigen: DNA Damage

Clonality: Monoclonal (15A3)

Host: Mouse

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361743

Additional Information

Immunogen: 8-hydroxy-guanosine-BSA and

Format: Protein G purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 15A3

Description: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2

Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2

Application Details

Application Notes: See assay instructions below.

Concentration: 0.65mg/ml

Buffer: Protein G-purified antibody in PBS, in 50% glycerol, 0.1% sodium azide.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Restrictions: For Research Use only

Images

DNARNA Damage (15A3), ischemic rat brain tissue. Courtesy of Dr. Yi Yang, U. New Mexico

Publications

Publications: Malins, Haimanot: "Major alterations in the nucleotide structure of DNA in cancer of the female breast." in: Cancer research, Vol. 51, Issue 19, pp. 5430-2, 1991 (PubMed).

Kvam, Tyrrell: "Artificial background and induced levels of oxidative base damage in DNA from human cells." in: Carcinogenesis, Vol. 18, Issue 11, pp. 2281-3, 1997 (PubMed).

Cui, Holmes, Greene et al.: "Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 14, Issue 7, pp. 955-67, 2000 (PubMed).

Kim, Murakami, Williams et al.: "Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells." in: Carcinogenesis, Vol. 24, Issue 2, pp. 235-41, 2003 (PubMed).

Bowers, Cool, Murphy et al.: "Oxidative stress in severe pulmonary hypertension." in: American journal of respiratory and critical care medicine, Vol. 169, Issue 6, pp. 764-9, 2004 (PubMed).

Kowluru, Atasi, Ho: "Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy." in: Investigative ophthalmology & visual science, Vol. 47, Issue 4, pp. 1594-9, 2006 (PubMed).

Pilger, Ruediger: "8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures." in: International archives of occupational and environmental health, Vol. 80, Issue 1, pp. 1-15, 2006 (PubMed).