anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term)

Details for Product anti-BBC3 Antibody No. ABIN361747
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Antigen
Synonyms BBC3, Puma, JFY-1, JFY1, PUMA, PUMA/JFY1
Epitope
C-Term
(99), (42), (14), (13), (12), (12), (11), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(258), (75), (56), (2), (1), (1)
Host
Rabbit
(221), (33), (13)
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
(11), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(248), (74), (51), (47), (36), (30), (10), (9), (6), (5), (3), (3), (2), (2)
Pubmed 3 references available
Quantity 25 μg
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Immunogen C-terminal amino acids of human PUMA
Specificity Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
Purification Affinity Purified
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Cellular Localization: Mitochondrion
Gene ID 27115
NCBI Accession NP_001120712
UniProt Q9BXH3
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Pathway
Application Notes Recommended Dilution: WB (1:1000), ICC/IF (1:100)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody PUMA (CT) , Human K562 cells, and Mouse 3T3 cell lysates.
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (2) PUMA (CT), K562 cells
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (3) Human breast carcinoma
Background publications Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Validation Images
Catalog No. ABIN361747
213.40 $
Plus shipping costs $45.00
Quantity
Price
25 μg
213.40 $

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