BCL2 Binding Component 3 (BBC3) antibody

Antigen: BCL2 Binding Component 3 (BBC3)

Synonyms: JFY1, PUMA, PUMA/JFY1, BBC3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN361748

Additional Information

Alternative name: Puma

Immunogen: C-terminal amino acides of human PUMA

Format: Affinity-purified

Description: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-

Specificity: Detects a 23 kD protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kd band may be seen in some instances, possibly corresponding to PUMA

Application Details

Application Notes: Suggested dilution 1: 1000 for Western blots, 1:100 for ICC.

Concentration: 1.0mg/mL

Buffer: Affinity purified in PBS with 0.02% Sodium Azide

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

PUMA (CT) , Human K562 cells, and Mouse 3T3 cell lysates

PUMA (NT), Human breast carcinoma

PUMA (CT), K562 cells