BIM antibody (BCL2-Like 11 (Apoptosis Facilitator))

Details for Product anti-BCL2L11 Antibody No. ABIN361751, Supplier: Log in to see
Antigen
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • LOC100345483
  • BCL2L11
  • Bcl2l11
Alternatives
anti-Rat (Rattus) BIM antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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9
6
6
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3
1
1
Host
Rabbit
278
29
16
9
7
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
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14
11
9
9
9
5
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3
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2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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33
28
17
7
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Options
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Available images

Immunogen Internal central amino acids of human Bim
Specificity Detects ~23 kDa.
Purification Protein A Purified
Alternative Name BIM/BOD (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Gene ID 10018
NCBI Accession NP_619527
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity
Application Notes
  • WB (1:400)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2.5 μg/ml of SPC-164 was sufficient for detection of Bim in 20 μg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN361751) Bim BOD (IN2), K562 Cells.
Western Blotting (WB) image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN361751) Bim Bod A549 Cell lysate 10ug WB 1 in 1000.
Background publications Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998 (PubMed).

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998 (PubMed).