PUMA antibody (N-Term)
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- Target See all PUMA (BBC3) Antibodies
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PUMA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Detects the N-terminal domain of PUMA ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
- Cross-Reactivity
- Fish, Human, Rainbow Trout, Rat, Salmon
- Purification
- Protein A Purified
- Immunogen
- N-terminal amino acids of human PUMA
- Top Product
- Discover our top product BBC3 Primary Antibody
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- Application Notes
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- WB (1:500)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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2 μg/ml of ABIN361753 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, (2012) (PubMed).
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Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, (2012) (PubMed).
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- PUMA (BBC3 Products)
- Synonyms
- BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
- Background
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
- Gene ID
- 27113
- NCBI Accession
- NP_001120712
- UniProt
- Q9BXH1
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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