BCL2 Binding Component 3 (BBC3) (N-Term) antibody

Antigen: BCL2 Binding Component 3 (BBC3)

Synonyms: JFY1, PUMA, PUMA/JFY1, BBC3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361753

Additional Information

Alternative name: PUMA

Gene ID: 27113

UniProt: Q9BXH1

Immunogen: N-terminal amino acids of human PUMA

Description: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms: BBC3, BCL2 binding component 3, p53 up regulated modulator of apoptosis, PUMA/JFY1

Characteristics: Accession Number: NP_001120712.1

Specificity: Detects a ~23kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kDa bands may be seen in some instances, possibly corresponding to PUMAbeta.

Application Details

Application Notes: 1:500 (WB), 1:100 (ICC)

Concentration: 1mg/mL

Purification: Affinity purified

Buffer: PBS

Preservative: 0.02% sodium azide

Storage: Store at -20° C. Shipping Temperature: Blue Ice or 4° C

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

PUMA (NT), in K562 human cell lysate.

Publications

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).