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BCL2 Binding Component 3 (BBC3) (N-Term) antibody
|Synonyms||JFY1, PUMA, PUMA/JFY1, BBC3|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|3 references available|
|Quantity||25µg (1mg/mL) (Variants)|
|Price||192.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||N-terminal amino acids of human PUMA|
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms: BBC3, BCL2 binding component 3, p53 up regulated modulator of apoptosis, PUMA/JFY1
|Characteristics||Accession Number: NP_001120712.1|
|Specificity||Detects a ~23kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kDa bands may be seen in some instances, possibly corresponding to PUMAbeta.|
|Application Notes||1:500 (WB), 1:100 (ICC)|
|Preservative||0.02% sodium azide|
|Storage||Store at -20° C. Shipping Temperature: Blue Ice or 4° C|
|Restrictions||For Research Use only|
|PUMA (NT), in K562 human cell lysate.|
Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).
Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).
Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).
|Hosts||Rabbit (62), Mouse (9), Goat (2)|
|Reactivities||Human (70), Mouse (Murine) (28), Rat (Rattus) (24), Cow (Bovine) (18), Dog (Canine) (18), Pig (Porcine) (18)|
|Applications||Western Blotting (WB) (59), ELISA (20), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (14), N-Term (7), AA 76-170 (4), Internal Region (2), AA 134-154 (1), AA 2-16 (1), BH3 Domain (1), Center (1)|