BBC3 antibody (BCL2 Binding Component 3) (N-Term)

Details for Product anti-BBC3 Antibody No. ABIN361754, Supplier: Log in to see
Antigen
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Alternatives
anti-Human BBC3 antibody for Western Blotting
Epitope
N-Term
95
61
19
18
16
12
10
9
9
8
5
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rainbow Trout (Oncorhynchus mykiss), Rat (Rattus)
290
123
106
17
1
1
1
Host
Rabbit
259
24
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
274
85
76
60
51
32
20
13
9
7
2
1
Options
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Available images

Immunogen N-terminal amino acids of human PUMA
Specificity Detects the N-terminal domain of PUMA ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
Purification Protein A Purified
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Gene ID 27114
NCBI Accession NP_001120712
UniProt Q9BXH2
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity
Application Notes
  • WB (1:500)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SPC-165 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (N-Term) (ABIN361754) PUMA (NT), in K562 human cell lysate.
Product cited in: Wakabayashi, Kosaka, Mori, Yamada: "Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, 2012 (PubMed).

Background publications Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Han, Flemington, Houghton, Gu, Zambetti, Lutz, Zhu, Chittenden: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).