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Phosphothreonine antibody

Details for Product No. ABIN361757, Supplier: Log in to see
Antigen
Reactivity
Chemical
88
1
1
Host
Rabbit
68
22
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
85
69
58
20
19
14
14
4
3
1
1
1
Options
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Immunogen Phosphothreonine conjugated to KLH
Specificity Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
Purification Protein A Purified
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Research Area Amino Acids, Signaling, Protein Modifications, Metabolism
Application Notes
  • WB (1:500)
  • ICC/IF (1:60)
  • ELISA (1:2000)
  • IP (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2 μg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Phosphothreonine antibody (ABIN361757) Phospho Threonine, mouse brain extract.
Background publications Goto, Kiyono, Tomono, Kawajiri, Urano, Furukawa, Nigg, Inagaki: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).