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Phosphotyrosine (0) antibody
| Antigen | Phosphotyrosine (0) |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Various Species |
| Application |
Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
|
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7 references available |
| Catalog no. | ABIN361758 |
| Quantity | 400ul (250) (Variants) |
| Price | 335.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 to 7 Business Days |
Additional Information
| Alternative name | Phospho-tyrosine |
| Immunogen | Phosphotyrosine conjugated to KLH |
| Format | Antiserum |
| Description | Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein |
| Specificity | Recognizes proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine. Species cross-reactivity: Multi-species. |
Application Details
| Application Notes | WB 2 ug/mL, ELISA 0.5 ug/ml, IP 10 ug/mg sample |
| Concentration | 250 |
| Buffer | Rabbit antiserum in TBS, 0.1%NaN3 |
| Storage | -20°C, 1 year+, shipped on cold packs or ambient |
| Restrictions | For Research Use only |
Publications
| Publications |
Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).
Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed). Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed). Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed). Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed). Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed). Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed). |
Alternatives
Alternatives for antigen "Phosphotyrosine (0)", type "Antibodies"
| Hosts | Mouse (138), Rabbit (30), Armenian Hamster (2), Goat (2) |
| Reactivities | Human (22), Rat (Rattus) (18), Dog (Canine) (13), Mouse (Murine) (13), Various Species (3), All Species (2), Chicken (1), Cow (Bovine) (1) |
| Applications | Western Blotting (WB) (139), Immunoprecipitation (IP) (87), ELISA (60), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Flow Cytometry (FACS) (20), Immunohistochemistry (IHC) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (26), HRP (18), FITC (14), PE (7), Agarose Beads (5), Alkaline Phosphatase (AP) (5), RPE (2), TAMRA (2), Rhodamine (1) |
