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Details for Product No. ABIN361758

Phosphotyrosine (pTyr) antibody

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»Alternatives All Species
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
Pubmed 7 references available
Catalog no. ABIN361758
Quantity 400 µL
378.40 $   Plus shipping costs $45.00
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Immunogen Phosphotyrosine conjugated to KLH
Isotype IgG
Specificity Recognizes proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Cross-Reactivity (Details) Does not cross-react with phosphoserine or threonine.
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies, and arehelpful in facilitating the identification of tyrosine kinase substrates.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications, Cell Signaling, Phosphorylation
Application Notes Recommended Dilution: 2 µg/mL (WB), 0.5 µg/mL (ELISA), 10 µg/mg (IP)
Restrictions For Research Use only
Concentration 250 µg/ mL
Buffer PBS pH 7, in 0.01 % sodium azide.
Preservative Sodium azide
Storage -20 °C
Background publications Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).

Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Alternatives for antigen "Phosphotyrosine", type "Antibodies"
Hosts (184), (47), (2)
Reactivities (167), (39), (25), (21), (16), (11), (5), (3), (2), (1)
Applications (173), (118), (92), (37), (31), (24), (18), (18), (14), (10), (3), (2), (1), (1), (1), (1)
Conjugates (29), (23), (19), (7), (7), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (2), (2), (1), (1), (1), (1), (1)