Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3) antibody

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 3 (HCN3)

Synonyms: CNG3, ACHM2, CCNC1, CCNCa, CNCG3, CCNCalpha, Hac3, Hcn4, Bcng4, BCNG-4, KIAA1535, MGC131493, CNGA3, HCN3

Clonality: Monoclonal (S141-28)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Catalog no.: ABIN361765

Additional Information

Characteristics: Detects ~90kDa. No cross-reactivity against other HCNs

Alternative name: HCN3

Gene ID: 15168

Swiss-Prot: O88705

Immunogen: Fusion protein amino acids 660-779 (C terminus) of mouse HCN3, accession number O88705

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S141-28

Description: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.
Synonyms: KIAA1535, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Specificity: ~90kDa. No cross reactivity against other HCNs. Species cross-reactivity: Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-306 was sufficient for detection of HCN3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

HCN3 (S141-28), Human hippocampus

Publications

Publications: Zong, Eckert, Yuan et al.: "A novel mechanism of modulation of hyperpolarization-activated cyclic nucleotide-gated channels by Src kinase." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34224-32, 2005 (PubMed).