KIR2.3 antibody

Antigen: KIR2.3

Clonality: Monoclonal (S25-35)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361771

Additional Information

Characteristics: Detects ~45kDa. No cross-reactivity against Kir2.1 or Kir2.2

Gene ID: 16520

Swiss-Prot: P52189

Immunogen: Fusion protein amino acids 390-445 of human Kir2.3, accession number NP_032453

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S25-35

Description: Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene (3-5).
Synonyms: IRK3, HIR, HIRK2, HRK1, KCNJ4, potassium inwardly rectifying channel subfamily J member 4

Specificity: ~45kDa. No cross reactivity against Kir2.1, or Kir2.2. Species cross-reactivity: Human, Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-312 was sufficient for detection of Kir2.3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

Kir2 3 (S25-35), human cell line mix

Kir2.3 (S25-35), Human hippocampus

Publications

Publications: Zobel, Cho, Nguyen et al.: "Molecular dissection of the inward rectifier potassium current (IK1) in rabbit cardiomyocytes: evidence for heteromeric co-assembly of Kir2.1 and Kir2.2." in: The Journal of physiology, Vol. 550, Issue Pt 2, pp. 365-72, 2003 (PubMed).

Muunoz, Vaidyanathan, Tolkacheva et al.: "Kir2.3 isoform confers pH sensitivity to heteromeric Kir2.1/Kir2.3 channels in HEK293 cells." in: Heart rhythm : the official journal of the Heart Rhythm Society, Vol. 4, Issue 4, pp. 487-96, 2007 (PubMed).

Panama, McLerie, Lopatin: "Heterogeneity of IK1 in the mouse heart." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 6, pp. H3558-67, 2007 (PubMed).