Kv3.1 antibody

Antigen: Kv3.1

Clonality: Monoclonal (S16B-8)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361772

Additional Information

Characteristics: Detects ~110kDa.

Gene ID: 25327

Swiss-Prot: P25122

Immunogen: Fusion protein amino acids 437-585 (C-terminus) or rat Kv3.1b, accession number NP_036988

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S16B-8

Description: Potassium voltage-gated channel, Shaw-related subfamily, member 1, also known as KCNC1 or Kv3.1, is a human gene. The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Kv3.1b has been extensively tested in the auditory regions of mammals, and the decline of Kv3.1b expression appears to correlate with the functional decline in the medial olivocochlear efferent system. Other research shows potential for Kv3.1b channels to be oxygen sensors.
Synonyms: Krc2 1, NGK2, KCNC1, voltage gated potassium channel subunit Kv3.1, Kv4

Specificity: ~110kDa. Species cross-reactivity: Human (weak), Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-313 was sufficient for detection of Kv3.1b in 10µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

KV3.1 (S16B-8), Human hippocampus

KV3.1(S16B-8), Rat Brain extract

KV3.1 (S16B-8), Mouse brain tissue, Amplifier

Publications

Publications: Osipenko, Tate, Gurney: "Potential role for kv3.1b channels as oxygen sensors." in: Circulation research, Vol. 86, Issue 5, pp. 534-40, 2000 (PubMed).

Zettel, Zhu, ONeill et al.: "Age-related decline in Kv3.1b expression in the mouse auditory brainstem correlates with functional deficits in the medial olivocochlear efferent system." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 8, Issue 2, pp. 280-93, 2007 (PubMed).

Xu, Cao, Xiao et al.: "The axon-dendrite targeting of Kv3 (Shaw) channels is determined by a targeting motif that associates with the T1 domain and ankyrin G." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 51, pp. 14158-70, 2007 (PubMed).