Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4)

Synonyms: TRP4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, CCE1, Trp4, Trrp4, STRPC4, TRPC4, TRPC5, Trpc4

Clonality: Monoclonal (S77-15)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN361774

Additional Information

Characteristics: Detects ~110kDa.

Alternative name: TrpC4

Gene ID: 84494

Swiss-Prot: O35119

Immunogen: Synthetic peptide amino acids 930-947 of rat TrpC4, accession number O35119

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S77-15

Description: Transient receptor potential cation channel, subfamily C, member 4, also known as TRPC4, is a human gene encoding a protein of the same name. They are expressed in smooth muscle and endothelial cells where they regulate membrane potential and calcium influx. TrpC4 is activated by G(q)/phospholipase C-coupled receptors, but the underlying mechanism remains elusive. Studies suggest TrpC4 contributes to axonal regeneration after nerve injury.
Synonyms: CCE1, HTRP4, STRPC4, TRP4, TRPC 4, transient receptor potential 4

Specificity: ~110kDa. Species cross-reactivity: Human, Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-315 was sufficient for detection of TrpC4 in 10µg of COS cell lysate transiently transfected with TrpC4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

TrpC4 (S77-15), Human hippocampus

TRPC4(S77-15), Human cell line mix

Publications

Publications: Wu, Huang, Richardson et al.: "TRPC4 in rat dorsal root ganglion neurons is increased after nerve injury and is necessary for neurite outgrowth." in: The Journal of biological chemistry, Vol. 283, Issue 1, pp. 416-26, 2007 (PubMed).

Otsuguro, Tang, Tang et al.: "Isoform-specific inhibition of TRPC4 channel by phosphatidylinositol 4,5-bisphosphate." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 10026-36, 2008 (PubMed).