Cavbeta4 antibody

Antigen: Cavbeta4

Clonality: Monoclonal (S10-7)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN361777

Additional Information

Characteristics: Detects ~50kDa. No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.

Gene ID: 785

Swiss-Prot: O00305

Immunogen: Synthetic peptide amino acids 458-474 of rat CavB4

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S10-7

Description: This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized .
Synonyms: CAB4, CACNLB4, EA5, EJM, Calcium channel voltage dependent beta 4 subunit

Specificity: ~50kDa. No cross reactivity against rat Cav

Sensitivity: 1 µg/mL of SMC-318 was sufficient for detection of Cavbeta4 in 10µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

Cavbeta4 (S10-7), T-CHO induced

CavBeta4 (S10-7), Human Hippocampus

Publications

Publications: Xie, Li, Han et al.: "Facilitation versus depression in cultured hippocampal neurons determined by targeting of Ca2+ channel Cavbeta4 versus Cavbeta2 subunits to synaptic terminals." in: The Journal of cell biology, Vol. 178, Issue 3, pp. 489-502, 2007 (PubMed).