Discs, Large Homolog 3 (Synapse-Associated Protein 102) (Sap102) (DLG3) antibody

Antigen: Discs, Large Homolog 3 (Synapse-Associated Protein 102) (Sap102) (DLG3)

Clonality: Monoclonal (S19-2)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN361790

Additional Information

Characteristics: Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.

Alternative name: SAP102

Gene ID: 58948

Swiss-Prot: Q62936

Immunogen: Fusion protein amino acids 1-120 ofd rat SAP102 (accession number Q62936)

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S19-2

Description: Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal. SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homolgy 3 domain and a guanylate kinase domain. All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway . SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains. In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
Synonyms: DLG3, MRX90, NEDLG, XLMR

Specificity: ~105 kDa. No cross-reactivity against PSD-95, SAP97, Chapsyn-110. Species Cross-Reactivity Mouse, Rat, Human.

Sensitivity: 1µg/ml was sufficient for detection of Sap102 in 10µg of rat brainlysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Application Details

Application Notes: WB: 1:1000 (ECL)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Neurology, Proliferation Markers

Restrictions: For Research Use only

Images

SAP102 (S19-2), Rat brain extract

Publications

Publications: Lau, Mammen, Ehlers et al.: "Interaction of the N-methyl-D-aspartate receptor complex with a novel synapse-associated protein, SAP102." in: The Journal of biological chemistry, Vol. 271, Issue 35, pp. 21622-8, 1996 (PubMed).

Mueller, Kistner, Kindler et al.: "SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo." in: Neuron, Vol. 17, Issue 2, pp. 255-65, 1996 (PubMed).

Cuthbert, Stanford, Coba et al.: "Synapse-associated protein 102/dlgh3 couples the NMDA receptor to specific plasticity pathways and learning strategies." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 10, pp. 2673-82, 2007 (PubMed).