Crystallin, alpha A (CRYAA) antibody

Antigen: Crystallin, alpha A (CRYAA)

Synonyms: CRYA1, HSPB4, Crya1, lop18, Acry-1, Crya-1, DAcry-1, AI323437, cryaa-A, CRYAA, fc06h04, MGC92036, zgc:92036, wu:fc06h04, alpha-a-crystallin

Clonality: Monoclonal (1H3-B8)

Host: Mouse

Reactivity: Human, Mouse (Murine), Cow (Bovine), Rat (Rattus)

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN361799

Additional Information

Characteristics: Detects alpha-crystallin at ~20kDa. Does not cross-react with alphaB-crystallin, betaL-crystallin, BetaH- crystallin, gamma-crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Alternative name: Alpha A Crystallin

Gene ID: 1409

Swiss-Prot: P02489

Immunogen: Native Alpha Crystallin

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1H3-B8

Description: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance . In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases.
Synonyms: Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4

Specificity: Detects alpha crystallin at ~20kDa. Species cross-reactivity: Human, Mouse, Cow, Rat. Other species not tested. Detects alpha crystallin complex (containing alpha A and alpha B subunits). Does not cross react with alpha B crystallin, beta-L crystallin, Beta-H crystallin, gamma crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Sensitivity: 0.5µg/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Application Details

Application Notes: 1:2000 (WB)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.2, in 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

Alpha A Crystallin (1H3.B8), alphaA (L) and AlphaB (R) crystallin