Lysine (lys) (acetylated) antibody

Catalog No. ABIN361803

Product Details

Target: Lysine (lys)

Binding Specificity: acetylated

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Specificity: Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.

Purification: Protein G Purified

Immunogen: Acetylated KLH

Clone: 7F8

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg of ABIN361802 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 μg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Lysine (lys)

Alternative Name: Lysine

Synonyms: lysine antibody, lys antibody

Target Type: Amino Acid

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).