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Phosphotyrosine (0) antibody
| Antigen | Phosphotyrosine (0) |
| Clonality | Monoclonal (13F9) |
| Host |
 Alternatives Armenian Hamster |
| Reactivity |
Alternatives All Species |
| Application |
Alternatives ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
|
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7 references available |
| Catalog no. | ABIN361806 |
| Quantity | 100ul |
| Price | 315.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 to 7 Business Days |
Additional Information
| Alternative name | Phospho-tyrosine |
| Immunogen | Phosphotyrosine conjugated to KLH |
| Isotype | IgG1 kappa (Matching secondary antibodies) |
| Clone | 13F9 |
| Description | Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein |
| Specificity | Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Does not cross react with phosphoserine or phosphothreonine. Species cross-reactivity: Not species specific. |
Application Details
| Application Notes | Working dilution 1:2000 to 1:10000 |
| Buffer | Clarified ascites containing 0.02%NaN3 |
| Storage | -20°C, 1 year+, shipped on cold packs or ambient |
| Restrictions | For Research Use only |
Publications
| Publications |
Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).
Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed). Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed). Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed). Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed). Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed). Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed). |
Alternatives
Alternatives for antigen "Phosphotyrosine (0)", type "Antibodies"
| Hosts | Mouse (138), Rabbit (31), Goat (2), Armenian Hamster (1) |
| Reactivities | Human (22), Rat (Rattus) (18), Dog (Canine) (13), Mouse (Murine) (13), Various Species (4), All Species (1), Chicken (1), Cow (Bovine) (1) |
| Applications | Western Blotting (WB) (140), Immunoprecipitation (IP) (87), ELISA (60), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (20), Flow Cytometry (FACS) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (26), HRP (18), FITC (14), PE (7), Agarose Beads (5), Alkaline Phosphatase (AP) (5), RPE (2), TAMRA (2), Rhodamine (1) |
