Phosphothreonine antibody

Details for Product No. ABIN361807, Supplier: Log in to see
Antigen
Reactivity
86
1
1
Host
Mouse
67
20
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
10
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
82
66
57
21
20
14
14
4
3
1
1
1
Options
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Immunogen Phosphothreonine conjugated to KLH
Clone 18F9
Isotype IgG1 kappa
Specificity Reacts with phosphothreonine, and detects the presence of phosphothreonine in proteins of both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphotyrosine.
Cross-Reactivity (Details) Does not cross-react with phosphoserine or phosphotyrosine.
Purification Ascites
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications, Cell Signaling
Application Notes Recommended Dilution: 1:2000 to 1:10000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Storage -20 °C
Background publications Goto, Kiyono, Tomono, Kawajiri, Urano, Furukawa, Nigg, Inagaki: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).