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Phosphothreonine (pThr) antibody
Alternatives ELISA, Western Blotting (WB)
|4 references available|
|Price||346.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Phosphothreonine conjugated to KLH|
|Description||Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).|
|Specificity||Reacts with phosphothreonine, and detects the presence of phosphothreonine in proteins of both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphotyrosine.|
|Application Notes||1:2000 to 1:10000. Not tested but should also work in RIA, flow cytometry, IHC, IP|
|Storage||Store at -20° C. Shipping Conditions: Blue Ice or 4° C|
|Research Area||Cell Signaling|
|Restrictions||For Research Use only|
Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).
Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).
Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).
Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).
|Hosts||Rabbit (37), Mouse (16)|
|Reactivities||Human (5), Dog (Canine) (4), Mouse (Murine) (4), Rat (Rattus) (4), All Species (2)|
|Applications||Western Blotting (WB) (50), ELISA (47), Immunoprecipitation (IP) (24), Immunohistochemistry (IHC) (2), Enzyme Immunoassay (EIA) (1), Functional Studies (Func) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||HRP (10), Biotin (8)|