Ubiquitin antibody

Details for Product No. ABIN361810
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Antigen
Synonyms
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si: ... show more
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, LOC100194618, UBIQ, Ubiquitin, LOC100282068 show less
Reactivity
Human, Rat (Rattus), Cow (Bovine)
(152), (108), (57), (56), (15), (15), (9), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host
Mouse
(138), (116), (4), (2), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (6), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(186), (129), (84), (66), (38), (32), (24), (23), (21), (10), (2), (1)
Pubmed 3 references available
Quantity 50 μg
Options
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Catalog No. ABIN361810
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Immunogen Native bovine ubiquitin conjugated to KLH
Clone 5B9-B3
Isotype IgG2a kappa
Specificity This antibody recognizes approx. 10 kDa corresponding to free ubiquitin
Sensitivity 1 µg/mL of SMC-160 was sufficient for detection of ubiquitin in 10 µg of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Background Synonyms:
Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of anubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27.
Gene ID 7314
NCBI Accession NP_061828
UniProt P62988
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids, Cell Signaling
Application Notes Recommended Dilution: 1:1000 (WB)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Ubiquitin antibody SMC 160, Ubiquitin (5B9 B3), human cell line muix.
Background publications Wilkinson: "Roles of ubiquitinylation in proteolysis and cellular regulation." in: Annual review of nutrition, Vol. 15, pp. 161-89, 1996 (PubMed).

Bonifacino, Weissman: "Ubiquitin and the control of protein fate in the secretory and endocytic pathways." in: Annual review of cell and developmental biology, Vol. 14, pp. 19-57, 1999 (PubMed).

General Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

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