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Ubiquitin antibody

Details for Product No. ABIN361816, Supplier: Log in to see
Antigen
  • CG11624
  • DmUb
  • DmUbi-p63E
  • Dmel\\CG11624
  • Ub
  • Ubi-P63e
  • Ubi-p
  • Ubi63E
  • Ubi63p
  • Ubip
  • Ubiq
  • Ubp
  • ubi
  • ubi-p63E
  • ubi63E
  • CG18282
  • CG32744
  • CR32744
  • DmUbi-p5E
  • Dmel\\CG32744
  • ubiquitin
  • si:dz180g5.5
  • si:busm1-180g5.5
  • LOC100194618
  • UBIQ
  • Ubiquitin
  • LOC100282068
  • polyubiquitin-B-like
  • Ubiquitin-63E
  • Ubiquitin-5E
  • transmembrane and ubiquitin-like domain containing 2
  • polyubiquitin
  • polyubiquitin containing 7 ubiquitin monomers
  • Ubiquitin
  • ubiquitin
  • ubiquitin B
  • LOC100716362
  • Ubi-p63E
  • Ubi-p5E
  • tmub2
  • LOC100194618
  • LOC100282068
  • LOC100190766
  • LOC100135798
  • UBB
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
322
195
188
183
59
47
38
36
31
28
27
27
27
26
26
25
24
13
8
5
3
3
3
2
2
2
2
2
1
1
1
1
1
Host
Mouse
222
206
5
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
18
15
13
13
13
12
8
8
8
8
8
8
8
8
8
8
6
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
365
238
186
134
123
117
66
33
22
13
13
12
7
3
1
1
1
Options
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Immunogen Native bovine ubiquitin, conjugated to KLH
Clone 6C11-B3
Isotype IgG2a kappa
Specificity Detects ~10 kDa.
Sensitivity 1 µg/mL of SMC-171 was sufficient for detection of ubiquitin in 10 µg of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Background Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (5).
Gene ID 281370
NCBI Accession NP_776558
UniProt P0CG53
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids
Pathways Mitotic G1-G1/S Phases
Application Notes
  • WB (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-171 was sufficient for detection of ubiquitin in 10 μg of Hela Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Ubiquitin antibody (ABIN361816) SMC 171, Ubiquitin (6C11 B3), human cvell line mix copy.
Product cited in: Antoni, Nuding, Weller, Gersemann, Ott, Wehkamp, Stange: "Human colonic mucus is a reservoir for antimicrobial peptides." in: Journal of Crohn's & colitis, 2013 (PubMed).

Background publications Bonifacino, Weissman: "Ubiquitin and the control of protein fate in the secretory and endocytic pathways." in: Annual review of cell and developmental biology, Vol. 14, pp. 19-57, 1999 (PubMed).

Wilkinson: "Roles of ubiquitinylation in proteolysis and cellular regulation." in: Annual review of nutrition, Vol. 15, pp. 161-89, 1996 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).