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HSP70 antibody

HSP70 Reactivity: Human WB, ELISA, IHC, IF, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361820
  • Target See all HSP70 Antibodies
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reactivity
    • 319
    • 197
    • 163
    • 71
    • 60
    • 53
    • 52
    • 52
    • 36
    • 31
    • 29
    • 29
    • 28
    • 23
    • 20
    • 18
    • 15
    • 13
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 206
    • 146
    • 13
    • 11
    • 11
    Rabbit
    Clonality
    • 228
    • 158
    Polyclonal
    Conjugate
    • 109
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    This HSP70 antibody is un-conjugated
    Application
    • 371
    • 159
    • 140
    • 122
    • 120
    • 109
    • 98
    • 66
    • 62
    • 61
    • 41
    • 33
    • 22
    • 21
    • 21
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    Detects a ~70 kDa. May cross-react with HSC70 at lower dilutions.
    Cross-Reactivity
    Beluga, Carp, Coral, Cow, Dog, Fish, Guinea Pig, Hamster, Human, Leishmania amazonensis, Monkey, Mouse, Pig, Plant, Rat, Shark, Sheep
    Purification
    Peptide Affinity Purified
    Immunogen
    Recombinant Full length HSP70 Protein
    Top Product
    Discover our top product HSP70 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • IP (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:1000 dilution of ABIN361820 was sufficient for detection of HSP70 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Woodford, Dunn, Blanden, Capriotti, Loiselle, Prodromou, Panaretou, Hughes, Smith, Ackerman, Haystead, Loh, Bourboulia, Schmidt, Marston Linehan, Bratslavsky, Mollapour: "The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding." in: Nature communications, Vol. 7, pp. 12037, (2018) (PubMed).

    Codonho, Costa, Peloso, Joazeiro, Gadelha, Giorgio: "HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics." in: Memórias do Instituto Oswaldo Cruz, (2017) (PubMed).

    Watanabe, Tsujimura, Taguchi, Tanaka: "HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis." in: Autophagy, Vol. 13, Issue 1, pp. 133-148, (2017) (PubMed).

    Silverstein, Ordanes, Wylie, Files, Milligan, Presley, Kavanagh: "Inducing Muscle Heat Shock Protein 70 Improves Insulin Sensitivity and Muscular Performance in Aged Mice." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 70, Issue 7, pp. 800-8, (2015) (PubMed).

  • Target
    HSP70 (Heat Shock Protein 70 (HSP70))
    Alternative Name
    HSP70 (HSP70 Products)
    Synonyms
    APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
    Background
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (1, 2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
    Gene ID
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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