Calnexin antibody (CANX)

Details for Product anti-CANX Antibody No. ABIN361839, Supplier: Log in to see
Antigen
  • zgc:63524
  • wu:fe06b12
  • clgn
  • clnx
  • cnx
  • CANX
  • CNX
  • IP90
  • P90
  • canx
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • PP90
  • CG11958
  • Dmel\CG11958
  • calnexin
  • Calnexin 99A
  • canx
  • CANX
  • CNX
  • LOC100283947
  • LOC100304606
  • canx-a
  • Canx
  • Cnx99A
Alternatives
anti-Human Calnexin antibody for Immunofluorescence (fixed cells)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Immunogen A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH
Specificity Detects the N-terminal domain of Calnexin ~90 kDa.
Purification Rabbit Antiserum
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Gene ID 403908
NCBI Accession NP_001003232
UniProt P24643
Pathways MAPK Signaling, Thyroid Hormone Synthesis
Application Notes
  • WB (1:5000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • IP (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:5000 dilution of SPC-127 was sufficient for detection of Calnexin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only
Format Liquid
Buffer Rabbit Antiserum
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-Calnexin antibody (CANX) (ABIN361839) Calnexin NT, Hela, Western Blotting.
Product cited in: Liu, Milner, Boppart, Ross, Kaufman: "β1D chain increases α7β1 integrin and laminin and protects against sarcolemmal damage in mdx mice." in: Human molecular genetics, Vol. 21, Issue 7, pp. 1592-603, 2012 (PubMed).

Background publications Janiszewski, Lopes, Carmo, Pedro, Brandes, Santos, Laurindo: "Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 280, Issue 49, pp. 40813-9, 2005 (PubMed).

Raggo, Rapin, Stirling, Gobeil, Smith-Windsor, OHare, Misra: "Luman, the cellular counterpart of herpes simplex virus VP16, is processed by regulated intramembrane proteolysis." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5639-49, 2002 (PubMed).

Schrag, Bergeron, Li, Borisova, Hahn, Thomas, Cygler: "The Structure of calnexin, an ER chaperone involved in quality control of protein folding." in: Molecular cell, Vol. 8, Issue 3, pp. 633-44, 2001 (PubMed).

Elagöz, Callejo, Armstrong, Rokeach: "Although calnexin is essential in S. pombe, its highly conserved central domain is dispensable for viability." in: Journal of cell science, Vol. 112 ( Pt 23), pp. 4449-60, 2000 (PubMed).

Rubio, Wenthold: "Calnexin and the immunoglobulin binding protein (BiP) coimmunoprecipitate with AMPA receptors." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 942-8, 1999 (PubMed).

Otteken, Moss: "Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 97-103, 1996 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Tjoelker, Seyfried, Eddy, Byers, Shows, Calderon, Schreiber, Gray: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, 1994 (PubMed).

Galvin, Krishna, Ponchel, Frohlich, Cummings, Carlson, Wands, Isselbacher, Pillai, Ozturk: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).