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Phosphotyrosine antibody (HRP)

Details for Product No. ABIN361843, Supplier: Log in to see
Antigen
Reactivity
All Species
142
107
72
29
28
25
10
9
8
3
1
Host
Rabbit
245
66
2
Clonality
Polyclonal
Conjugate
HRP
28
24
16
15
6
6
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
245
145
142
120
115
96
70
64
45
27
13
4
2
2
1
1
1
1
1
Options
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Immunogen Phosphotyrosine conjugated to KLH
Specificity Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Purification Protein A Purified
Target Type Amino Acid
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
Research Area Amino Acids, Signaling, Protein Modifications, Metabolism
Application Notes
  • WB (1:500)
  • IHC (1:100)
  • ICC/IF (1:50)
  • ELISA (1:2000)
  • IP (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Goto, Kiyono, Tomono, Kawajiri, Urano, Furukawa, Nigg, Inagaki: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed).

Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).