Phosphotyrosine antibody (Biotin)

Details for Product No. ABIN361844
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Antigen
Reactivity
Chemical
(150), (59), (44), (27), (27), (23), (8), (7), (7), (3), (1)
Host
Rabbit
(205), (62), (2)
Clonality
Polyclonal
Conjugate
Biotin
(27), (26), (21), (15), (9), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(204), (123), (100), (73), (70), (52), (33), (22), (15), (12), (10), (2), (2), (1), (1), (1)
Pubmed 7 references available
Quantity 400 μL
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Immunogen Phosphotyrosine conjugated to KLH
Specificity Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
Purification Rabbit immunoglobulin
Target Type Amino Acid
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Recommended Dilution: Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.01 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Phosphotyrosine antibody (Biotin) Phospho Tyrosine, Human Breast Cancer Tissue
Background publications Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed).

Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).

Validation Images
Catalog No. ABIN361844
456.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
456.50 $

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