DnaJ (HSP40, HDJ 2) antibody

Antigen: DnaJ (HSP40, HDJ 2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB)

Catalog no.: ABIN361857

Additional Information

Alternative name: DNAJ

Immunogen: Recombinant DNAJ of Chlamydomonas reinhardtii

Format: Antiserum

Description: DNAJ is a prokaryotic heat shock induced protein from the bacterium E.coli, which is under control of the htpr regulatory protein (1). DNAJ interacts with the chaperone Hsp70-like DNAK protein to disassemble a protein complex found at a phage lambda

Specificity: ~40kDa. Species cross-reactivity: Chlamydomonas reinhardtii.

Application Details

Application Notes: Recommended dilution for WB 1:5000

Buffer: Lyophilized rabbit serum (For reconstitution add 200uL of sterile water)

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Restrictions: For Research Use only

Publications

Publications: Szyperski, Pellecchia, Wall et al.: "NMR structure determination of the Escherichia coli DnaJ molecular chaperone: secondary structure and backbone fold of the N-terminal region (residues 2-108) containing the highly conserved J domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 24, pp. 11343-7, 1994 (PubMed).

Pellecchia, Szyperski, Wall et al.: "NMR structure of the J-domain and the Gly/Phe-rich region of the Escherichia coli DnaJ chaperone." in: Journal of molecular biology, Vol. 260, Issue 2, pp. 236-50, 1996 (PubMed).

Goehre, Ossenbuehl, Cruevecoeur et al.: "One of two alb3 proteins is essential for the assembly of the photosystems and for cell survival in Chlamydomonas." in: The Plant cell, Vol. 18, Issue 6, pp. 1454-66, 2006 (PubMed).