DnaK antibody

Antigen: DnaK

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB)

Catalog no.: ABIN361858

Additional Information

Immunogen: Recombinant DNAK of Chlamydomonoas reinhardtii

Format: Antiserum

Description: The prokaryotic Hsp70 protein family includes DNA K, HSCA (Hsc66), and HscC (Hsc62) (1). DNAK interacts with DNAJ and GrpE heat shock protein chaperones to fold substrate proteins in an ATP-driven cycle (2). These proteins are also involved in cell protection from environmental stress and other functions which are vital for a living cell. And like eukaryotic Hsp70 homologs, DNAK participates in the assembly/disassembly of protein complexes (3, 4)

Specificity: Detects ~70kDa. Species cross-reactivity: Chlamydomonas reinhardtii.

Application Details

Application Notes: Recommended dilution for WB 1:5000

Concentration: 1mg/ml

Buffer: Lyophilized Rabbit Serum (for reconstitution, add 200uL of sterile water)

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Restrictions: For Research Use only

Publications

Publications: Pelham: "Speculations on the functions of the major heat shock and glucose-regulated proteins." in: Cell, Vol. 46, Issue 7, pp. 959-61, 1986 (PubMed).

Zylicz, LeBowitz, McMacken et al.: "The dnaK protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 21, pp. 6431-5, 1983 (PubMed).

Hightower: "Cultured animal cells exposed to amino acid analogues or puromycin rapidly synthesize several polypeptides." in: Journal of cellular physiology, Vol. 102, Issue 3, pp. 407-27, 1980 (PubMed).

Goehre, Ossenbuehl, Cruevecoeur et al.: "One of two alb3 proteins is essential for the assembly of the photosystems and for cell survival in Chlamydomonas." in: The Plant cell, Vol. 18, Issue 6, pp. 1454-66, 2006 (PubMed).