Heat Shock Protein 101 (Hsp101) antibody

Antigen: Heat Shock Protein 101 (Hsp101)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Maize/Corn (Zea mays)

Application: Western Blotting (WB)

Catalog no.: ABIN361863

Additional Information

Alternative name: Hsp101

Immunogen: 15aa peptide sequence from the C-terminus of mainze Hsp101. Gene accession number AF077337.

Format: Antiserum

Description: Hsps/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (2, 3). Hsp100 is one such major class that acts as a chaperone. Hsp101/ClpB is a member of this Hsp100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of Hsp100 protein family are also expressed during seed development. Hsp101 protein is both nuclear and cytoplasmic-localized (1, 4).

Specificity: ~101kDa. Species cross-reactivity: Zea mays. No reactivity to other cereal Hsp101.

Application Details

Application Notes: Recommended dilution for WB 1:2000 with ECL or AP

Buffer: Lyophilized rabbit serum (For reconstitution add 200uL of sterile water)

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Heat Shock Proteins

Restrictions: For Research Use only

Images

Hsp101 (ClpB CT) Maize

Publications

Publications: Nieto-Sotelo, Kannan, Martuinez et al.: "Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue." in: Gene, Vol. 230, Issue 2, pp. 187-95, 1999 (PubMed).

Nieto-Sotelo, Martuinez, Ponce et al.: "Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth." in: The Plant cell, Vol. 14, Issue 7, pp. 1621-33, 2002 (PubMed).

Evenepoel, Maes, Wilmer et al.: "Detoxifying capacity and kinetics of the molecular adsorbent recycling system. Contribution of the different inbuilt filters." in: Blood purification, Vol. 21, Issue 3, pp. 244-52, 2003 (PubMed).