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HSP70 antibody (Heat Shock Protein 70)

Details for Product anti-HSP70 Antibody No. ABIN361868, Supplier: Log in to see
Antigen
  • HSP70
  • HEAT SHOCK PROTEIN 70
  • HEAT SHOCK PROTEIN 70-7
  • HSC70-7
  • K9P8.5
  • K9P8_5
  • chloroplast heat shock protein 70-2
  • cpHsc70-2
  • F19K16.12
  • F19K16_12
  • LOC100305036
  • hsc70
  • CG31354
  • Hsp70Bb
  • hsp70B
  • hsp70Bb-prime
  • DmelCG5834
  • CG5834
  • APG-2
  • HS24/P52
  • HSPH2
  • RY
  • hsp70
  • hsp70RY
  • hsc71
  • Hsp70
  • Hsp70-1
  • Hsp70.1
  • hsp68
  • Hsp110
  • irp94
  • HSPA1
  • HSP70B'
  • HSPA6
  • ARABIDOPSIS HEAT SHOCK PROTEIN 70
  • ATHSP70
  • heat shock protein 70
  • hsp70-5
  • heat shock 70 kD protein cognate
  • heat shock protein 70-2
  • Heat shock protein 70
  • heat shock protein 70
  • CG5834 gene product from transcript CG5834-RA
  • heat shock 70kDa protein 1A
  • heat shock 70kDa protein 2
  • heat shock 70kDa protein 4
  • heat shock cognate protein 70a
  • heat shock protein 1B
  • heat shock protein 4
  • heat shock protein 70-4
  • heat shock protein1
  • Protein HSP-70
  • LOC692373
  • CPHSC70-2EAT SHOCK PROTEIN 70-2
  • AT1G79920
  • HSP70
  • PCC7424_2419
  • Isop_1041
  • CGB_C3390W
  • Bacsa_1698
  • dnaK-B
  • LOC100305036
  • Hsp70Bbb
  • HSPA1A
  • HSPA2
  • HSPA4
  • hsc70a
  • Hspa1b
  • Hspa4
  • hsp1
  • hsp-70
Reactivity
Arabidopsis, Bacteria, Fish, Fungi (Fusarium), Mammalian, Plant
616
424
423
197
184
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149
114
110
103
100
96
86
86
75
58
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50
39
38
35
26
24
20
18
18
17
8
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4
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3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
376
307
40
18
Clonality
Polyclonal
Conjugate
This HSP70 antibody is un-conjugated
35
34
25
23
22
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20
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20
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20
20
11
9
7
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5
5
5
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5
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4
4
3
3
2
Application
Western Blotting (WB)
657
288
279
272
256
235
149
106
72
62
50
26
18
11
9
4
2
2
1
Options
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Immunogen KLH-conjugated synthetic peptide conserved in all known HSP70 and HSC70 proteins
Specificity Detects ~70 kDa.
Purification Protein A Purified
Alternative Name HSP70 (HSP70 Antibody Abstract)
Background HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed, HSP 70 is highly related to HSC70 (>90 % sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Research Area Heat Shock Proteins
Application Notes
  • WB (1:1000)
  • IP (1:1000)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SPC-311 was sufficient for detection of HSP70/HSC70 in 1 mg wet plant sample extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized, PBS pH 7.4. For reconstitution add 100 μL of sterile water.
Storage -20 °C
Product cited in: Santos, Janissen, Toledo, Beloti, Azzoni, Cotta, Souza: "Characterization of the TolB-Pal trans-envelope complex from Xylella fastidiosa reveals a dynamic and coordinated protein expression profile during the biofilm development process." in: Biochimica et biophysica acta, Vol. 1854, Issue 10 Pt A, pp. 1372-1381, 2015 (PubMed).

Background publications Brown, Hong-Brown, Doxsey, Welch: "Molecular chaperones and the centrosome. A role for HSP 73 in centrosomal repair following heat shock treatment." in: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 833-40, 1996 (PubMed).

Boorstein, Ziegelhoffer, Craig: "Molecular evolution of the HSP70 multigene family." in: Journal of molecular evolution, Vol. 38, Issue 1, pp. 1-17, 1994 (PubMed).

Bork, Sander, Valencia: "An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7290-4, 1992 (PubMed).

Rothman: "Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells." in: Cell, Vol. 59, Issue 4, pp. 591-601, 1990 (PubMed).

DeLuca-Flaherty, McKay, Parham, Hill: "Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis." in: Cell, Vol. 62, Issue 5, pp. 875-87, 1990 (PubMed).