Heat Shock 70kDa Protein 7 (HSP70B) (HSPA7) (C-Term) antibody

Details for Product No. ABIN361872
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Antigen
Synonyms HSP70B
Epitope
C-Term
(6), (2), (1), (1), (1)
Reactivity
Chlamydomonas reinhardtii (C. reinhardtii)
(9), (1), (1)
Host
Rabbit
(10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(10), (7), (2), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN361872
382.80 $
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Immunogen Mature Hsp70B protein expressed with N- and C- terminal hexahistidine tags in E.coli purified with Ni-NTA
Isotype IgG
Specificity Detects approx. 70 kDa.
Alternative Name Hsp70B
Background Synonyms:
Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, HSPA1, HSPA1A, HSPA1B
Hsp70 genes encode abundant heat-inducible 70 kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O % identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteinsand synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins.When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed, hsp 70 is highly related to hsc70 (>90 % sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock.
Gene ID 5722220
NCBI Accession XP_001696432
UniProt A8HYV3
Research Area Heat Shock Proteins
Application Notes Recommended Dilution: 1:10000 (WB)
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Background publications Schroda, Vallon, Wollman et al.: "A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition." in: The Plant cell, Vol. 11, Issue 6, pp. 1165-78, 1999 (PubMed).

Schroda, Vallon, Whitelegge et al.: "The chloroplastic GrpE homolog of Chlamydomonas: two isoforms generated by differential splicing." in: The Plant cell, Vol. 13, Issue 12, pp. 2823-39, 2001 (PubMed).

Liu, Willmund, Whitelegge et al.: "J-domain protein CDJ2 and HSP70B are a plastidic chaperone pair that interacts with vesicle-inducing protein in plastids 1." in: Molecular biology of the cell, Vol. 16, Issue 3, pp. 1165-77, 2005 (PubMed).

Willmund, Hinnenberger, Nick et al.: "Assistance for a chaperone: Chlamydomonas HEP2 activates plastidic HSP70B for cochaperone binding." in: The Journal of biological chemistry, Vol. 283, Issue 24, pp. 16363-73, 2008 (PubMed).

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