HSP70 antibody (Heat Shock Protein 70) (C-Term)

Details for Product anti-HSP70 Antibody No. ABIN361872, Supplier: Log in to see
Antigen
  • HSP70
  • HEAT SHOCK PROTEIN 70
  • HEAT SHOCK PROTEIN 70-7
  • HSC70-7
  • K9P8.5
  • K9P8_5
  • chloroplast heat shock protein 70-2
  • cpHsc70-2
  • F19K16.12
  • F19K16_12
  • LOC100305036
  • hsc70
  • CG31354
  • Hsp70Bb
  • hsp70B
  • hsp70Bb-prime
  • DmelCG5834
  • CG5834
  • APG-2
  • HS24/P52
  • HSPH2
  • RY
  • hsp70
  • hsp70RY
  • hsc71
  • Hsp70
  • Hsp70-1
  • Hsp70.1
  • hsp68
  • Hsp110
  • irp94
  • HSPA1
  • HSP70B'
  • HSPA6
  • ARABIDOPSIS HEAT SHOCK PROTEIN 70
  • ATHSP70
  • heat shock protein 70
  • hsp70-5
  • heat shock 70 kD protein cognate
  • heat shock protein 70-2
  • Heat shock protein 70
  • heat shock protein 70
  • CG5834 gene product from transcript CG5834-RA
  • heat shock 70kDa protein 1A
  • heat shock 70kDa protein 2
  • heat shock 70kDa protein 4
  • heat shock cognate protein 70a
  • heat shock protein 1B
  • heat shock protein 4
  • heat shock protein 70-4
  • heat shock protein1
  • Protein HSP-70
  • LOC692373
  • CPHSC70-2EAT SHOCK PROTEIN 70-2
  • AT1G79920
  • HSP70
  • PCC7424_2419
  • Isop_1041
  • CGB_C3390W
  • Bacsa_1698
  • dnaK-B
  • LOC100305036
  • Hsp70Bbb
  • HSPA1A
  • HSPA2
  • HSPA4
  • hsc70a
  • Hspa1b
  • Hspa4
  • hsp1
  • hsp-70
Epitope
C-Term
41
34
31
21
20
20
17
15
15
12
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Algae, Plant
620
424
423
197
184
173
169
149
114
110
103
100
96
86
86
75
58
54
50
39
38
35
26
24
20
19
18
18
17
8
8
6
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
379
308
40
18
Clonality
Polyclonal
Conjugate
This HSP70 antibody is un-conjugated
35
35
25
24
23
21
21
20
20
20
20
20
20
20
20
20
11
9
7
6
5
5
5
5
5
5
4
4
4
3
3
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
657
291
282
275
255
233
149
109
72
62
50
26
18
9
9
4
2
2
1
Options
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Immunogen Mature HSP70B protein expressed with N- and C- terminal hexahistidine tags in E.coli, purified with Ni-NTA
Specificity Detects ~70 kDa.
Purification Rabbit Antiserum
Alternative Name HSP70 (HSP70 Antibody Abstract)
Background HSP70B (heat shock protein 70B) is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas chloroplasts. It interacts with HSP90C, CGE1, CDJ2 and VIPP1 (1-3). It has been shown that using Chlamydomonas reinhardtii as plant model organism the alga encodes a HEP homolog (termed HEP2) that is localized to the stroma (4). HEP2 is expressed constitutively as a low abundance protein with an apparent molecular mass of ~21 kDa. In cell extracts HEP2 interacts with HSP70B in an ATP-dependent fashion. Coexpression of HSP70B with HEP2 in E. coli yields high levels of CGE1- binding competent HSP70B, which also displayed ATPase activity (4). Inactive HSP70B was more prone to proteolysis than active HSP70B. Although inactive HSP70B interacts with HEP2, it is not activated. Active HSP70B remains active for 48 h in the absence of HEP2, suggesting that HEP2 was not involved in maintaining HSP70B in an active state. However, it was found that some HSP70B expressed as a fusion protein with an N-terminal extension was activated when HEP2 was present during cleavage of the fusion protein, suggesting that in vivo HEP2 might be required for renewed folding of HSP70B after transit peptide cleavage (4). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Gene ID 5722220
NCBI Accession XP_001696432
UniProt A8HYV3
Research Area Heat Shock Proteins
Application Notes
  • WB (1:10000)
  • optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized rabbit Antiserum. For reconstitution add 100 μL of sterile water.
Storage -20 °C
Background publications Willmund, Hinnenberger, Nick, Schulz-Raffelt, Mühlhaus, Schroda: "Assistance for a chaperone: Chlamydomonas HEP2 activates plastidic HSP70B for cochaperone binding." in: The Journal of biological chemistry, Vol. 283, Issue 24, pp. 16363-73, 2008 (PubMed).

Liu, Willmund, Whitelegge, Hawat, Knapp, Lodha, Schroda: "J-domain protein CDJ2 and HSP70B are a plastidic chaperone pair that interacts with vesicle-inducing protein in plastids 1." in: Molecular biology of the cell, Vol. 16, Issue 3, pp. 1165-77, 2005 (PubMed).

Schroda, Vallon, Whitelegge, Beck, Wollman: "The chloroplastic GrpE homolog of Chlamydomonas: two isoforms generated by differential splicing." in: The Plant cell, Vol. 13, Issue 12, pp. 2823-39, 2001 (PubMed).

Schroda, Vallon, Wollman, Beck: "A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition." in: The Plant cell, Vol. 11, Issue 6, pp. 1165-78, 1999 (PubMed).