»
Antibodies
»
anti-Heat Shock Protein 90 (HSP90) antibody
Heat Shock Protein 90 (HSP90) antibody
| Antigen |
|
| Synonyms |
swo1, hsp90, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (44), Mouse (Murine) (14), Rabbit (11), Rat (Rattus) (11), Chicken (10), Achlya ambisexualis (8), Cow (Bovine) (4), Guinea Pig (3), Plasmodium falciparum (3), Achlya (2), Insect cells (Sf9) (2), Pig (Porcine) (2), Salmon (Salmonidae) (2), Wheat germ (2), Dog (Canine) (1), Frog (1), Monkey (1), Sheep (Ovine) (1)
|
|
Application
|
 Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|
| Catalog no. |
ABIN361874 |
| Quantity |
100uL (Variants) |
| Price |
339.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 to 7 Business Days |
|
Alternative name
|
Hsp90
|
|
Immunogen
|
C-terminal238 amino acids of Hsp90C expressed with N- and C-terminal hexahistidine tags in E.coli, purified with Ni-NTA.
|
|
Format
|
Antiserum
|
|
Description
|
Hsp90C is a nuclear-encoded, chloroplast-targeted chaperone of the Hsp90 family. In the chloroplast, it is localized to the soluble stroma fractin, but also to thylakoids and low-density membranes containing inner envelopes. Hsp90C is expressed under basal conditions and is strongly induced by heat shock and moderately by light. It forms dimers that interact with Hsp70B. Hsp90C displays a weak ATPase activity that is inhibited by radicicol (1).
|
|
Specificity
|
Detects ~83kDa. Hsp90C is easily degraded and the antibody detects these degradation fragmensts. Species cross-reactivity: Chlamydomonas reinhardtii. Other species not yet tested.
|
|
Application Notes
|
Recommended dilution for WB 1:3000 with ECL
|
|
Buffer
|
Lyophilized rabbit serum. (For reconstitution add 100uL of sterile water).
|
|
Storage
|
-20°C, 1 year+, shipped on cold packs or ambient
|
|
Research Area
|
Cancer, Heat Shock Proteins
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Willmund, Schroda: "HEAT SHOCK PROTEIN 90C is a bona fide Hsp90 that interacts with plastidic HSP70B in Chlamydomonas reinhardtii." in: Plant physiology, Vol. 138, Issue 4, pp. 2310-22, 2005 (PubMed).
|
Alternatives for antigen "Heat Shock Protein 90 (HSP90)", type "Antibodies"
|
Hosts
|
Mouse (37), Rabbit (23), Rat (5)
|
|
Reactivities
|
Human (44), Mouse (Murine) (14), Rabbit (11), Rat (Rattus) (11), Chicken (10), Achlya ambisexualis (8), Cow (Bovine) (4), Guinea Pig (3), Plasmodium falciparum (3), Achlya (2), Insect cells (Sf9) (2), Pig (Porcine) (2), Salmon (Salmonidae) (2), Wheat germ (2), Dog (Canine) (1), Frog (1), Monkey (1), Sheep (Ovine) (1)
|
|
Applications
|
Western Blotting (WB) (54), Immunohistochemistry (IHC) (33), Immunoprecipitation (IP) (29), ELISA (27), Flow Cytometry (FACS) (8), Immunocytochemistry (ICC) (5), Enzyme Immunoassay (EIA) (4), Radioimmunoassay (RIA) (4), Immunofluorescence (IF) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3)
|
|
Conjugates
|
Biotin (3), PE (2)
|
