Myocyte Enhancer Factor 2A (MEF2A) (pThr319) antibody

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Antigen
  • MEF2A
  • xmef2a
  • ADCAD1
  • RSRFC4
  • RSRFC9
  • mef2
  • A430079H05Rik
  • adcad1
  • rsrfc4
  • rsrfc9
  • sl2
  • mef2a
  • wu:fd19d02
  • myocyte enhancer factor 2A
  • myocyte-specific enhancer factor 2A
  • myocyte-specific enhancer factor 2a
  • myocyte enhancer factor 2a
  • myocyte enhancer factor 2aa
  • MEF2A
  • EDI_092490
  • EDI_038250
  • Smp_161530
  • Smp_129430
  • mef2a
  • Mef2a
  • mef2aa
Alternatives
anti-Human Myocyte Enhancer Factor 2A antibody for Immunofluorescence (fixed cells)
Epitope
pThr319
42
36
24
21
18
15
15
12
11
11
10
10
9
7
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
268
169
138
18
13
13
12
11
11
8
8
6
5
3
3
2
2
Host
Rabbit
218
52
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF)
174
114
91
78
67
26
16
9
7
5
2
2
1
Options
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Immunogen Peptide sequence around phosphorylation site of pThr319 (V-T-T (p) -P-S) derived from Human MEF2A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of MEF2A only when phosphorylated at Thr319.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed bychromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MEF2A (MEF2A Antibody Abstract)
Background The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain
Molecular Weight 54 kDa
NCBI Accession NP_001124
UniProt Q02078
Pathways Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
Application Notes Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Dang: "c-Myc target genes involved in cell growth, apoptosis, and metabolism." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 1-11, 1999 (PubMed).

Martin, Miano, Hustad, Copeland, Jenkins, Olson: "A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 1647-56, 1994 (PubMed).

Henson, Walker: "The structure of nucleohistone. Hydrodynamic behaviour at high ionic strength." in: European journal of biochemistry / FEBS, Vol. 22, Issue 1, pp. 1-4, 1971 (PubMed).