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Antibody
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anti-B-Cell CLL-lymphoma 2 BCL2 pThr56
B-Cell CLL/lymphoma 2 (BCL2) (pThr56) antibody
| Antigen |
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| Synonyms |
Bcl-2, bcl-2, BCL2 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (125), Mouse (Murine) (51), Rat (Rattus) (50), Dog (Canine) (36), Cow (Bovine) (30), Pig (Porcine) (30), Rabbit (17), Chicken (15), Horse (Equine) (15), Cat (Feline) (2), Gerbil (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (84), Immunofluorescence (IF) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Immunoprecipitation (IP) (38), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), ELISA (19), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2)
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| Catalog no. |
ABIN361993 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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BCL-2 (Phospho-Thr56)
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Swiss-Prot
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P10415
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-T P -P-H).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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BCL-2 (Phospho-Thr56) (Human) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
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Hosts
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Rabbit (102), Mouse (28), Goat (4), Sheep (3)
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Reactivities
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Human (125), Mouse (Murine) (51), Rat (Rattus) (50), Dog (Canine) (36), Cow (Bovine) (30), Pig (Porcine) (30), Rabbit (17), Chicken (15), Horse (Equine) (15), Cat (Feline) (2), Gerbil (1)
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Applications
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Western Blotting (WB) (84), Immunofluorescence (IF) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Immunoprecipitation (IP) (38), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), ELISA (19), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2)
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Conjugates
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PE (3), Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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pSer70 (7), pThr56 (6), BH3 Domain Specific (1)
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