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B-Cell CLL/lymphoma 2 (BCL2) (pSer70) antibody

Antigen

B-Cell CLL/lymphoma 2 (BCL2)
Synonyms Bcl-2, bcl-2, BCL2
Epitope
Alternatives

pSer70
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC), ELISA
Catalog no. ABIN361995
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name BCL-2 (Phospho-Ser70)
Swiss-Prot P10415
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-S P -P-L).
Isotype IgG  (Matching secondary antibodies)
Specificity BCL-2 (Phospho-Ser70) (Human) antibody detects endogenous levels of BCL-2 only when phosphorylated at serine 70.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes IHC: 1:50~1:100 ELISA: 1:10000
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
Hosts Rabbit (102), Mouse (28), Goat (4), Sheep (3)
Reactivities Human (125), Mouse (Murine) (51), Rat (Rattus) (50), Dog (Canine) (36), Cow (Bovine) (30), Pig (Porcine) (30), Rabbit (17), Chicken (15), Horse (Equine) (15), Cat (Feline) (2), Gerbil (1)
Applications Western Blotting (WB) (85), Immunofluorescence (IF) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Immunoprecipitation (IP) (38), Flow Cytometry (FACS) (33), Immunohistochemistry (IHC) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), ELISA (18), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (EM) (2)
Conjugates PE (3), Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
Epitopes pThr56 (7), pSer70 (6), BH3 Domain Specific (1)