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p53 antibody (pSer37)

TP53 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362054
  • Target See all p53 (TP53) Antibodies
    p53 (TP53) (Tumor Protein P53 (TP53))
    Binding Specificity
    • 51
    • 41
    • 40
    • 39
    • 32
    • 30
    • 28
    • 25
    • 24
    • 24
    • 23
    • 20
    • 20
    • 20
    • 18
    • 18
    • 16
    • 16
    • 14
    • 13
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    pSer37
    Reactivity
    • 890
    • 270
    • 220
    • 110
    • 45
    • 34
    • 31
    • 18
    • 10
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 678
    • 228
    • 6
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 612
    • 303
    • 1
    Polyclonal
    Conjugate
    • 511
    • 49
    • 39
    • 26
    • 25
    • 24
    • 24
    • 24
    • 22
    • 22
    • 22
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This p53 antibody is un-conjugated
    Application
    • 720
    • 336
    • 254
    • 216
    • 208
    • 150
    • 124
    • 123
    • 119
    • 62
    • 61
    • 32
    • 20
    • 17
    • 13
    • 12
    • 12
    • 10
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Specificity
    The antibody detects endogenous level of p53 only when phosphorylated at serine 37.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pSer37 (L-P-S (p) -Q-A) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
    Top Product
    Discover our top product TP53 Primary Antibody
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Guan, Li, Yang, Huang, Zheng, Zhang, Li, Liu: "Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-? plus IFN-? on polystyrene and the role of p53 activation." in: Biomaterials, Vol. 33, Issue 26, pp. 6162-71, (2012) (PubMed).

  • Target
    p53 (TP53) (Tumor Protein P53 (TP53))
    Alternative Name
    p53 (TP53 Products)
    Synonyms
    BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
    Background
    P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes
    Molecular Weight
    53 kDa
    NCBI Accession
    NP_000537
    UniProt
    P04637
    Pathways
    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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