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beta Catenin antibody (Catenin, beta) (pSer45)

Details for Product anti-Catenin, beta Antibody No. ABIN362089, Supplier: Log in to see
Antigen
  • armadillo
  • beta-catenin
  • Bfc
  • Catnb
  • CHBCAT
  • CTNNB
  • ctnnb
  • ctnnb1
  • id:ibd2058
  • Mesc
  • MRD19
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
Epitope
pSer45
39
36
31
29
22
21
19
15
15
14
13
13
12
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
348
257
240
17
15
11
10
10
10
9
5
5
4
3
1
Host
Rabbit
305
46
4
Clonality
Polyclonal
Conjugate
This beta Catenin antibody is un-conjugated
11
11
9
9
9
9
9
9
9
9
9
8
1
1
1
1
Application
Western Blotting (WB)
236
105
101
79
44
39
38
16
13
9
5
2
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pThr 41, pSer45 (A-T-T (p) -T-A-P-S (p) -L-S) derived from Human b-Catenin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of b-Catenin only when phosphorylated at threonine 41/serine 45.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name beta-Catenin
Background Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
Molecular Weight 92 kDa
NCBI Accession NP_001091
UniProt P35222
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Provost, McCabe, Stern, Lizardi, DAquila, Rimm: "Functional correlates of mutation of the Asp32 and Gly34 residues of beta-catenin." in: Oncogene, Vol. 24, Issue 16, pp. 2667-76, 2005

Provost, Yamamoto, Lizardi, Stern, DAquila, Gaynor, Rimm: "Functional correlates of mutations in beta-catenin exon 3 phosphorylation sites." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 31781-9, 2003

Amit, Hatzubai, Birman, Andersen, Ben-Shushan, Mann, Ben-Neriah, Alkalay: "Axin-mediated CKI phosphorylation of beta-catenin at Ser 45: a molecular switch for the Wnt pathway." in: Genes & development, Vol. 16, Issue 9, pp. 1066-76, 2002

Sakanaka: "Phosphorylation and regulation of beta-catenin by casein kinase I epsilon." in: Journal of biochemistry, Vol. 132, Issue 5, pp. 697-703, 2002

Liu, Li, Semenov, Han, Baeg, Tan, Zhang, Lin, He: "Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism." in: Cell, Vol. 108, Issue 6, pp. 837-47, 2002