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AKT2 antibody (pSer474)
AKT2
Reactivity: Human, Mouse, Rat
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-AKT2 Antibody
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Target
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AKT2
(V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
Binding Specificity
All epitopes for AKT2 antibodies
pSer474
Reactivity
All reactivities for AKT2 antibodies
Human, Mouse, Rat
Host
All hosts for AKT2 antibodies
Rabbit
Clonality
All clonalities for AKT2 antibodies
Polyclonal
Conjugate
All conjugates for AKT2 antibodies
This AKT2 antibody is un-conjugated
Application
All applications for AKT2 antibodies
Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer474 (Q-F-S (p) -Y-S) derived from Human Akt2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for AKT2
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Target
AKT2
(V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
Alternative Name
Akt2 (AKT2 Products )
Synonyms
AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody
Background
General protein kinase capable of phosphorylating several known proteins.
Molecular Weight
60 kDa
UniProt
P31751
Pathways
PI3K-Akt Signaling , RTK Signaling , AMPK Signaling , TLR Signaling , Cellular Glucan Metabolic Process , Regulation of Carbohydrate Metabolic Process , Hepatitis C , VEGF Signaling
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