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LIM Domain Kinase 1 antibody (LIMK1) (pThr508)

Details for Product anti-LIMK1 Antibody No. ABIN362108, Supplier: Log in to see
Antigen
  • GB16654
  • LIMK
  • LIMK-1
  • LIMK1
  • limk1
  • xlimk1
Alternatives
anti-Human LIM Domain Kinase 1 antibody for Western Blotting
Epitope
pThr508
42
21
20
13
13
8
7
6
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
205
83
68
3
2
2
1
Host
Rabbit
144
72
2
Clonality
Polyclonal
Conjugate
This LIM Domain Kinase 1 antibody is un-conjugated
7
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
174
89
66
54
34
27
18
12
11
6
4
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pThr508 (R-Y-T (p) -V-V) derived from Human LIMK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK1 (LIMK1 Antibody Abstract)
Background Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
Molecular Weight 72 kDa
NCBI Accession NP_002305
UniProt P53667
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Pathways Caspase Cascade in Apoptosis
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Li, Ke, Li, Liu, Zhu, Li: "DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, 2009 (PubMed).

Background publications Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch, Gill: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).