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CDK2 antibody (pThr160)
CDK2
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-CDK2 Antibody
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Target
See all CDK2 Antibodies
CDK2
(Cyclin-Dependent Kinase 2 (CDK2))
Binding Specificity
All epitopes for CDK2 antibodies
pThr160
Reactivity
All reactivities for CDK2 antibodies
Human, Mouse, Rat
Host
All hosts for CDK2 antibodies
Rabbit
Clonality
All clonalities for CDK2 antibodies
Polyclonal
Conjugate
All conjugates for CDK2 antibodies
This CDK2 antibody is un-conjugated
Application
All applications for CDK2 antibodies
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
The antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pThr160 (T-Y-T (p) -H-E) derived from Human CDK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for CDK2
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Target
CDK2
(Cyclin-Dependent Kinase 2 (CDK2))
Alternative Name
CDK2 (CDK2 Products )
Synonyms
p33(CDK2) antibody, A630093N05Rik antibody, wu:fa10c02 antibody, zgc:56598 antibody, zgc:77684 antibody, eg1 antibody, CDKN2 antibody, cdk2L antibody, cyclin dependent kinase 2 antibody, cyclin-dependent kinase 2 antibody, cyclin-dependent kinase 2 S homeolog antibody, CDK2 antibody, Cdk2 antibody, cdk2 antibody, cdk2.S antibody
Background
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Molecular Weight
34 kDa
NCBI Accession
NP_001789
UniProt
P24941
Pathways
PI3K-Akt Signaling , Cell Division Cycle , Mitotic G1-G1/S Phases , DNA Replication , M Phase , Synthesis of DNA
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