HDAC2 antibody (pSer394)

Catalog No. ABIN362214

Product Details anti-HDAC2 Antibody

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Binding Specificity: pSer394

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer394 (E-D-S (p) -G-D) derived from Human HDAC2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: HDAC2 (HDAC2 Products)

Synonyms: HD2 antibody, RPD3 antibody, YAF1 antibody, D10Wsu179e antibody, Yy1bp antibody, mRPD3 antibody, HISTONE DEACETYLASE antibody, T1N24.9 antibody, T1N24_9 antibody, histone deacetylase 2 antibody, histone deacetylase 2 antibody, histone deacetylase 2 S homeolog antibody, HDAC2 antibody, Hdac2 antibody, hdac2.S antibody, HDA2 antibody

Background: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes

Molecular Weight: 60 kDa

NCBI Accession: NP_001518

UniProt: Q92769

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection