Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) (pSer71) antibody

Antigen: Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1)

Synonyms: MIG5, TC-25, p21-Rac1, MGC111543

Epitope: pSer71

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN362231

Additional Information

Alternative name: Rac1 (Phospho-Ser71)

Swiss-Prot: P15154

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Rac1 around the phosphorylation site of serine 71 (P-L-S P -Y-P).

Isotype: IgG  (Matching secondary antibodies)

Specificity: Rac1 (Phospho-Ser71) (Human) antibody detects endogenous levels of Rac1 only when phosphorylated at serine 71.

Application Details

Application Notes: WB:1:500~1000

Concentration: 50µg/50µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20°C. Avoid repeat freeze-thaw cycles.

Restrictions: For Research Use only