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NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (pSer22)

Details for Product anti-NFKBIE Antibody No. ABIN362247, Supplier: Login to see
Antigen
Alternatives
anti-Human NFKBIE antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
pSer22
38
13
13
5
3
2
2
2
2
2
2
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
97
54
36
5
1
Host
Rabbit
97
2
Clonality
Polyclonal
Conjugate
This NFKBIE antibody is un-conjugated
4
4
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
64
39
33
18
15
9
3
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer22 (I-E-S (p) -L-R) derived from Human IkB-e. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of IkB-e only when phosphorylated at serine 22.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name IkB-epsilon (NFKBIE Antibody Abstract)
Background Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Molecular Weight 40 kDa
NCBI Accession NP_004547
UniProt O00221
Pathways NF-kappaB Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications McDevitt: "Discovering the role of the major histocompatibility complex in the immune response." in: Annual review of immunology, Vol. 18, pp. 1-17, 2000 (PubMed).

Shirane, Hatakeyama, Hattori et al.: "Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28169-74, 1999 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

Brown, Gerstberger, Carlson et al.: "Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1485-8, 1995 (PubMed).

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